human mesenchymal stem cells elevate cd4+cd25+cd127low/- regulatory t cells of asthmatic patients via heme oxygenase-1.

نویسندگان

jian-guo li department of respirology, sun yat-sen memorial hospital, sun yat-sen university, respiratory disease research institute of sun yat-sen university, guangzhou, guangdong province, people’s republic of china

yong-xun zhuan-sun department of respirology, sun yat-sen memorial hospital, sun yat-sen university, respiratory disease research institute of sun yat-sen university, guangzhou, guangdong province, people’s republic of china

bing wen department of respirology, sun yat-sen memorial hospital, sun yat-sen university, respiratory disease research institute of sun yat-sen university, guangzhou, guangdong province, people’s republic of china

hao wu department of emergency, sun yat-sen memorial hospital, sun yat-sen university, respiratory disease research institute of sun yat-sen university, guangzhou, guangdong province, people’s republic of china

چکیده

up-regulation of cd4+cd25+cd127low/- regulatory t cells (tregs) is a new target in the treatment of asthma. human bone marrow mesenchymal stem cells can up-regulate cd4+cd25+cd127low/- regulatory t cells in vitro,  meanwhile, heme oxygenase-1 (ho-1) plays an important role in the development and maintenance of cd4+cd25+ regulatory t cells. however the mechanism has not yet been adequately understood. hence, we wondered what effect of heme oxygenase-1 made on regulation of cd4+cd25+cd127low/-  regulatory t cells mediated by mesenchymal stem cells. peripheral blood mononuclear cells isolated from asthmatic patients and healthy controls were co-cultured with human bone marrow mesenchymal stem cells which were pretreated with hemin (the revulsive of heme oxygenase-1), protoporphyrin ⅸ zinc (the inhibitor of heme oxygenase-1) and saline. the expression of heme oxygenase-1 in mscs was enhanced by hemin and inhibited by protoporphyrin  zinc in vitro. overexpression of heme oxygenase-1 elevated the proportion of cd4+cd25+cd127low/- regulatory t cells in cd4+ t cells, meanwhile, inhibition of heme oxygenase-1 decreased the proportion of cd4+cd25+cd127low/- regulatory t cells in cd4+ t cells as compared with mesenchymal stem cells alone. taken together, these data demonstrated that heme oxygenase-1 contributed to the up-regulation of cd4+cd25+cd127low/- regulatory t cells mediated by mesenchymal stem cells in asthma.

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Human mesenchymal stem cells elevate CD4+CD25+CD127low/- regulatory T cells of asthmatic patients via heme oxygenase-1.

Up-regulation of CD4+CD25+CD127low/- regulatory T cells (Tregs) is a new target in the treatment of asthma. Human bone marrow mesenchymal stem cells can up-regulate CD4+CD25+CD127low/- regulatory T cells in vitro, meanwhile, heme oxygenase-1 (HO-1) plays an important role in the development and maintenance of CD4+CD25+ regulatory T cells. However the mechanism has not yet been adequately unders...

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عنوان ژورنال:
iranian journal of allergy, asthma and immunology

جلد ۱۲، شماره ۳، صفحات ۲۲۸-۲۳۵

کلمات کلیدی
[ ' a s t h m a ' , ' h e m e o x y g e n a s e ' , 1 , ' m e s e n c h y m a l s t r o m a l c e l l s ' , ' t ' , ' l y m p h o c y t e s ' , ' r e g u l a t o r y ' ]

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